Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessarily in this order: forks converge until ...

Our lab studies the mechanism of eukaryotic chromosome replication. Chromosomes are the carriers of the genetic and epigenetic information and faithful chromosome replication is of fundamental ...

Embryonic stem (ES) cells are pluripotent stem cells that can produce all cell types of an organism. ES cells proliferate rapidly and have been thought to experience high levels of intrinsic ...

Replication forks formed at bacterial origins often encounter template roadblocks in the form of DNA adducts and frozen protein-DNA complexes, leading to replication-fork stalling and inactivation.

When an SSB appears, polymerase epsilon approaches the nick before retracing its steps thanks to its exonuclease, allowing fork reversal to take place. The nick is repaired as an SSB, and replication ...

Every time a cell divides, it must copy its entire genome so that each daughter cell inherits a complete set of DNA. During that process, enzymes known as polymerases race along the DNA to copy its ...

The human genome consists of 3 billion base pairs, and when a cell divides, it takes about seven hours to complete making a copy of its DNA. That's almost 120,000 base pairs per second. At that ...

Despite the importance of DNA replication, numerous aspects of this process are still poorly understood. One fundamental question is: how do replication forks efficiently progress through chromatin?

Mouse ES cells present active origin firing and replication forks that progress slowly throughout S phase. These features are integral aspects of genome replication ensuring genome integrity in ES ...