Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq ® ...

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

To assess COLD-PCR enrichment, many different assays can be applied, either in combination with COLD-PCR (real-time COLD-PCR [35]) or downstream, in order to analyze enriched genetic variations. The ...