The 3′ overhang and no-overhang templates used for in vitro DNA replication reactions were constructed by ligating oligonucleotides to the plasmid vVA25 following its linearization with SapI. To ...
New findings suggest the end-replication problem, an old standby of biology textbooks, is twice as intricate as once thought. Half a century ago, scientists Jim Watson and Alexey Olovnikov ...